Actin-Myosin ATPase Assay

The actin-activated myosin ATPase assay is a key biochemical method used to measure the enzymatic activity of myosin motor proteins in the presence of actin filaments. This assay provides critical insights into the kinetic properties of myosin, its interaction with actin, and how various conditions, regulatory proteins, or small molecules affect motor function.

In this assay, myosin hydrolyzes ATP into ADP and inorganic phosphate (Pi), and the rate of phosphate release is quantified—either colorimetrically or fluorometrically. The presence of filamentous actin significantly stimulates myosin's ATPase activity, and this stimulation forms the basis for assessing actomyosin interactions and motor activity.

Applications include:

• Characterizing myosin isoform-specific activity

• Comparing wild-type vs. mutant proteins

• Investigating actin modifications (e.g., acetylated, arginylated)

• Screening for motor protein inhibitors or activators

At Andex Biotech, we supply highly purified, functionally active actin, myosin (full-length and fragments), and modified actin isoforms, validated for ATPase assays. Whether you're working with skeletal, cardiac, or non-muscle myosins, our reagents are optimized for reliable and reproducible ATPase measurements.