Recombinant gamma actin (ACTG) Recombinant Human Protein

41.67 kDa

Recombinant Human gamma actin.

Fully functional.

Polymerises and depolymerises.

Activates ATPase activity of myosin.

Purity: >95%.

No tags.

Sequence:

EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF

Postranslational modifications:

Acetyaltion of N-terminus

Methylation of His73

Gamma-actin (γ-actin) is one of the six isoforms of actin found in eukaryotic cells. It is encoded by the ACTG1 gene in humans and is primarily found in non-muscle cells, where it plays a crucial role in maintaining the cytoskeleton, supporting cell shape, and facilitating cellular processes such as migration and intracellular transport. γ-actin is particularly abundant in stress fibers and is essential for maintaining the mechanical stability of cells.

Key Functions of Gamma-actin:

1. Cytoskeletal Integrity: Helps maintain cell shape and mechanical stability.

2. Cell Motility and Migration: Plays a role in directed cell movement, especially during development and wound healing.

3. Intracellular Transport: Assists in the trafficking of vesicles and organelles.

4. Cell-Cell and Cell-Matrix Adhesion: Contributes to maintaining connections between cells and their environment.

5. Response to Cellular Stress: Involved in remodeling the cytoskeleton in response to mechanical stress.

Structural Features:

• Composed of 375 amino acids with a molecular weight of ~42 kDa.

• Exists in both monomeric (G-actin) and polymeric (F-actin) forms, dynamically assembling into actin filaments.

• Highly conserved across species, indicating its fundamental role in cell function.

Gamma-actin vs. Other Actin Isoforms:

• Beta-actin (β-actin): More concentrated in the leading edge of migrating cells and involved in rapid cytoskeletal remodeling.

• Alpha-actin (α-actin): Found mainly in muscle cells and essential for muscle contraction.

• Gamma-actin (γ-actin): More involved in maintaining the mechanical stability of the cell and stress fiber formation.

Clinical Relevance:

• Hearing Loss: Mutations in the ACTG1 gene are associated with nonsyndromic hearing loss, as γ-actin is critical for the function of hair cells in the inner ear.

• Cytoskeletal Disorders: Abnormalities in γ-actin expression or function can lead to cytoskeletal defects, impacting cell stability and motility.

• Cancer and Fibrosis: Dysregulation of γ-actin can contribute to abnormal cell migration, influencing cancer metastasis and fibrotic diseases.

Since γ-actin is essential for cellular architecture and function, it plays a significant role in both normal physiology and disease pathology.