Recombinant gamma actin (ACTG), labelled with Pyrene, Recombinant Human Protein
€350.00
Labelled with pyrene at cysteine 374.
41.67 kDa
Human gamma actin.
Fully functional.
Polymerises and depolymerises.
Activates ATPase activity of myosin.
Purity: >95%.
No tags.
Sequence:
EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF
Postranslational modifications:
Acetyaltion of N-terminus
Methylation of His73
Gamma-actin (γ-actin) is one of the six isoforms of actin found in eukaryotic cells. It is encoded by the ACTG1 gene in humans and is primarily found in non-muscle cells, where it plays a crucial role in maintaining the cytoskeleton, supporting cell shape, and facilitating cellular processes such as migration and intracellular transport. γ-actin is particularly abundant in stress fibers and is essential for maintaining the mechanical stability of cells.
Key Functions of Gamma-actin:
Cytoskeletal Integrity: Helps maintain cell shape and mechanical stability.
Cell Motility and Migration: Plays a role in directed cell movement, especially during development and wound healing.
Intracellular Transport: Assists in the trafficking of vesicles and organelles.
Cell-Cell and Cell-Matrix Adhesion: Contributes to maintaining connections between cells and their environment.
Response to Cellular Stress: Involved in remodeling the cytoskeleton in response to mechanical stress.
Structural Features:
Composed of 375 amino acids with a molecular weight of ~42 kDa.
Exists in both monomeric (G-actin) and polymeric (F-actin) forms, dynamically assembling into actin filaments.
Highly conserved across species, indicating its fundamental role in cell function.
Gamma-actin vs. Other Actin Isoforms:
Beta-actin (β-actin): More concentrated in the leading edge of migrating cells and involved in rapid cytoskeletal remodeling.
Alpha-actin (α-actin): Found mainly in muscle cells and essential for muscle contraction.
Gamma-actin (γ-actin): More involved in maintaining the mechanical stability of the cell and stress fiber formation.
Clinical Relevance:
Hearing Loss: Mutations in the ACTG1 gene are associated with nonsyndromic hearing loss, as γ-actin is critical for the function of hair cells in the inner ear.
Cytoskeletal Disorders: Abnormalities in γ-actin expression or function can lead to cytoskeletal defects, impacting cell stability and motility.
Cancer and Fibrosis: Dysregulation of γ-actin can contribute to abnormal cell migration, influencing cancer metastasis and fibrotic diseases.
Since γ-actin is essential for cellular architecture and function, it plays a significant role in both normal physiology and disease pathology.