Custom Protein Services
Precision-Engineered Recombinant Actin Proteins
High-purity, functional recombinant actin for research, drug discovery, and biotechnology applications.
At Andex Biotech, we deliver reliable, high-quality custom protein production with a deep specialization in actin and actin-related proteins. Whether you need standard skeletal muscle actin, cytoplasmic β-actin, γ-actin, or engineered actin variants, we provide end-to-end solutions tailored to your exact specifications.
Why Recombinant Actin Matters
Actin is one of the most conserved and essential proteins in eukaryotic cells. It plays critical roles in:
Cell motility and structure
Muscle contraction
Cytoskeletal dynamics
Intracellular transport
Wound healing and immune response
Cancer metastasis and neurodegenerative diseases
Our recombinant actin proteins are produced to match or exceed the quality of native sources while offering superior batch-to-batch consistency, purity, and customization options that natural extracts cannot provide.
Our Custom Protein Production Services
Recombinant Actin Expression & Purification
Full-length wild-type actins (α, β, γ isoforms)
Muscle-specific and non-muscle actins
Fluorescently labeled actins (e.g., Alexa Fluor, Cy3/Cy5)
Biotinylated, His-tagged, or GST-tagged variants
Site-directed mutants and engineered actin constructs
Actin-binding protein complexes
Expression Systems
Yeast systems
Mammalian cells (HEK293) – for native-like modifications
Insect cells (Sf9/Sf21) – superior for proper folding and post-translational modifications
Quality Standards
95–99% purity (validated by SDS-PAGE, SEC-HPLC)
Functional validation (polymerization assays, pyrene-actin fluorescence, binding assays)
Endotoxin testing (<0.1 EU/µg)
Mass spectrometry confirmation
Lyophilized or stabilized liquid formulations
End-to-End Custom Project Workflow
Consultation – Discuss your project requirements, sequence, tags, and intended applications.
Gene Synthesis & Cloning – Optimized codon usage for your chosen expression host.
Expression Screening – Small-scale tests to determine best conditions.
Scale-Up Production – From milligrams to gram-scale quantities.
Purification & Characterization – Multi-step chromatography and rigorous QC.
Delivery – With detailed Certificate of Analysis and technical support.
Typical turnaround: 4–8 weeks depending on complexity and scale.
Applications We Support
In vitro motility assays
Cytoskeletal drug screening
Structural biology (Cryo-EM, X-ray crystallography)
Actin polymerization kinetics studies
Development of actin-targeted therapeutics
Cell biology and mechanobiology research
Biosensor and nanotechnology development
Why Researchers Choose Index Biotech
Actin Specialists: Deep expertise in the unique challenges of actin expression (solubility, stability, and polymerization control).
High Functionality: Our recombinant actins demonstrate excellent polymerization competence and interaction with ABPs (actin-binding proteins).
Flexibility: From research-grade to preclinical material.
Confidentiality & IP Protection: Complete protection of your sequences and project details.
Responsive Support: Scientists available for technical consultation.
Ready to advance your actin research?
Contact our team today for a free consultation and quote.
Tell us your target sequence, desired modifications, and quantity – we’ll deliver proteins you can trust.